![]() Gram stain results reflect differences in cell wall composition. Knowing the Gram reaction of a clinical isolate can help the health care professional make a diagnosis and choose the appropriate antibiotic for treatment. Since then, the Gram stain procedure has been widely used by microbiologists everywhere to obtain important information about the bacterial species they are working with. This very commonly used staining procedure was first developed by the Danish bacteriologist Hans Christian Gram in 1882 (published in 1884) while working with tissue samples from the lungs of patients who had died from pneumonia. You will learn how to prepare bacterial cells for staining, and learn about the gram staining technique. Some examples of differential stains are the Gram stain, acid-fast stain, and endospore stain. Differential stains use more than one stain, and cells will have a different appearance based on their chemical or structural properties. Scientists will often choose to perform a differential stain, as this allows them to gather additional information about the bacteria they are working with. Isolated and imaged by Muntasir Alam, University of Dhaka, Department of Microbiology in 2007. Image 1: Microscopic view of Bacillus (rod) shaped bacteria simple stained with crystal violet. ![]() Basic stains, having a positive charge, bind strongly to negatively charged cell components such as bacterial nucleic acids and cell walls. The single dye used here in our lab is methylene blue, a basic stain. In a simple stain, a bacterial smear is stained with a solution of a single dye that stains all cells the same color without differentiation of cell types or structures. The purpose of staining is to increase the contrast between the organisms and the background so that they are more readily seen in the light microscope. Living bacteria are almost colorless, and do not present sufficient contrast with the water in which they are suspended to be clearly visible. Simple stains can be used to determine a bacterial species’ morphology and arrangement, but they do not give any additional information. Some stains commonly used for simple staining include crystal violet, safranin, and methylene blue. One type of staining procedure that can be used is the simple stain, in which only one stain is used, and all types of bacteria appear as the color of that stain when viewed under the microscope. Trending Questions What is the role of the menisci in the knee? What is the least prevalent mode of inheritance? What is the difference between duckweed and water lily? What did Sir Alexander Fleming greatest contribute to Science? Is tendonitis a bone injury? What are the factors that affect the structural basis of the skin? All of the following belong to feedback systems which control homeostasis except? Meiosis 2 during which the nuclear envelope reforms around genetic material in four new haploid cells? What is the air that may be exhaled in above the normal volume in a forced exhalation called? What resources are made from organisims that lived a long time time ago? What are treatments for anosmia? Fluid in the left pleural cavity tends to gravitate down to the? In how many ways are plant and animal cells different? What are the two main divisions of the nervous system and what makes up both of them? What do you call the science that study fungi? What produces microtubules during cell division in animals? Which hand is the best right or left? What would you do if you were stranded on an island for four years? Which of the following nutrient sources yields more than 4 kcalories per gram.\)
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